Using Agarose Resin chromatography allows for a versatile, prepacked column that enables small-scale, high-resolution size exclusion chromatography for preparation, characterization, and analysis of proteins and other biomolecules.
Quenchers are a substance that can decrease or end an effect or process. They are often used to absorb the excitation energy emitted by fluorophores (i.e., fluorescence), which signals that another process has taken place. Most re-emit much of the energy as visible light. Others, called dark quenchers, do not have native fluorescence and instead emit the energy as heat. Examples of common quenchers include iodide ions, molecular oxygen and acrylamide..
Immunoassays are tests that detect the presence of a specific molecule in a sample using antibody-antigen binding reactions. Antibodies bind to the specific structure of a particular antigen, making immunoassays highly specific: the antibody will only bind to a specific structure of a particular antigen. This makes antibodies effective reagents for detecting target molecules. As a result, immunoassays are a fundamental tool for hospitals, life science research and industry laboratories. Immunoassays come in a range of formats, and can be used to assess disease, track proteins, and detect environmental contamination.
DBCO click chemistry is a type of reaction that has underpinned recent advances in biomedical research, as well as pharmaceuticals and biotech. Copper(I)-free click chemistry, such as DBCO click reactions, enable fast and specific conjugation of target molecules under aqueous conditions and do not require toxic catalysts.
Poly deoxythymine (poly dt) is a molecule made up of a string of deoxythymidines that are connected via 3' to 5' phosphodiester linkages.
In other words, poly dt is a long chain of thymine nucleotides, which can be up to 20,000 nucleotides long. Also known as polythymidine, polythymidylic acid, and poly T, amongst other names, poly dt is a single-stranded sequence commonly used to purify messenger RNA (mRNA)..
The magnetic cell separation technique: Executing cell separation by magnetic activation has been a trusted technique by scientists for decades. Cell sorting is ubiquitously used in research and clinical settings where a target cell of interest needs to be isolated from a heterogenous mixture such as serum or plasma. It is used in several scientific disciplines such as immunology, where it helps identify cells present during immune responses, or in cancer research elucidating tissue environment of tumors.
A constant magnetic force across the whole working volume is key to consistency in biomagnetic separation processes. This ensures that all beads in the suspension experience the same force. Classical magnetic separators can't provide these conditions because the magnetic force they generate decreases with distance.
Quick Background on Cell Sorting/Cell Isolation
Researchers use magnetic activated cell isolation, also known as macs cell sorting or macs cell separation, to enrich a specific cell type from a mixed population. Scientists or companies sort or isolate cells so they can study or grow colonies of a single type of cell. They may use that type of cell for a specific type of functional assay crucial to that cell type or they might be interested in stem cells. Many labs use flow cytometry, also known as FACS (fluorescence activated cell sorting). This is a specialized and expensive technique that often resides in a core facility at a research institution. MACS has emerged as a cheaper alternative for cell sorting. Both these technologies use the highly specific antibody-antigen interaction to probe cells by their surface antigens by their specific antibody. Magnetic bead cell isolation has a more simple protocol and components than FACS, the process is briefly described below.
Biodetection and biosensors are widely used for diagnosing disease or infection, point-of-care monitoring and treatment, detecting toxins, environmental monitoring, forensics and research. Biosensing technology has a crucial role to play in future biomedicine and healthcare. Biodetection is a broad term that encompasses the global strategies in place for the detection of biological threats such as pathogens, infectious diseases, and biological weapons. In particular, portable biosensing instruments such as lab-on-chip technologies are opening up new possibilities for biodetection systems identifying outbreaks of infectious pathogens.
Flow cytometry interpretation can be challenging, particularly when conducting multiparametric analyses that typically create a large quantity of data points. Fluorescent activated cell sorting (FACS), a derivative of flow cytometry, has become an essential tool for modern life science and biomedical laboratories. It is one of two main methods for cell sorting, alongside magnetic bead separation. FACS is a method for rapidly analyzing cells based on multiple criteria simultaneously, and then physically sorting them based on this analysis. The results of the process are usually represented by dot plots: two or three-dimensional scatter plots, or sometimes a histogram. However, these plots can be tricky to interpret, so here are a few tips to help get you started.