It is vitally important to understand the process and all of the variables of the process when scaling up a biomagnetic separation in a **magnetic separation rack**. If you do not understand the details of your process, you will throw away your initial investment you made in **validating your initial process** and jeopardize the product’s time to market.

This post is about biomagnetic separation with a **magnetic separation rack**, and how to scale-up this process. If you are interested in this topic, download our free ebook **The Basic Guide to Scale-up Biomagnetic Separation Processes**:

## Understanding how to maintain constant magnetic force

In **biomagnetic separation processes**, magnetic beads move because the magnetic force is greater than the drag force on the beads due to the buffer viscosity. Therefore, the key parameter to understand is the **magnetic force**. The magnetic force depends both on how the magnetic field changes spatially (i.e. the magnetic field gradient) and on the **strength of the magnetization of the magnetic beads**.

The **two conditions that must be met** in order for a constant magnetic force to be maintained in biomagnetic separation processes are:

- The magnetic field needs to
**vary linearly with the distance**of the beads from the magnet. - The beads should be
**magnetically saturated**so that the field is high enough (e.g. B < 0.1 T for magnetite).

## Advanced separation systems fulfill both conditions

The newer, more **advanced homogeneous biomagnetic separation systems** such as SEPMAG fulfill these two conditions in virtually any volume desired. This is accomplished because the homogeneous systems are designed with a **cylindrical geometry** comprised of a **constant radial magnetic field** in the core. In these systems, the gradient is adjusted so that the **magnetic field is over 0.1 T** everywhere except in a small area around the axis.

Because of this constant force in the advanced systems, SEPMAG guarantees that **no less than 93% of the beads are saturated** when the process starts. After a few seconds, all of the beads have moved out of the central region and are in optimal conditions. The **initial 7% of beads not in optimal conditions** are considered the **practical limit of the device**.

**Advanced homogeneous separation systems** such as SEPMAG are therefore, **easy to scale up** because these conditions are determined in smaller volumes and remain the same when scaled up to larger volumes. When using a **standard magnetic separation rack**, volume matters because the beads feel different forces relative to their distance from the magnets. Scaling up a process with standard devices forces the company to **conduct validation and quality control experiments** in order to find the new correct parameters in the larger volumes.

Don't forget to check these posts from our blog in order to get a deeper insight into the **scaling-up of biomagnetic separation processes**:

- Why the force over different volume bottles is constant in advanced biomagnetic separation systems?
- How the scaling-up of biomagnetic separation process may increase profitability?
- What is the Best Way to Scale Up When Different Volumes are Required?

Check www.sepmag.eu/ebooks to access to FREE eBooks on the subject, or contact us. We will be glad to help you to achieve an efficient magnetic bead separation process!