Blog

Posted on Thu, Aug 24, 2017

CHIP assay

A CHIP assay is short for Chromatin Immunoprecipitation assay. This technique is used to gain insight into the region of the genome a particular protein is associated with. To do this the CHIP assay captures information about the protein-DNA interactions in the histones of genomic DNA. The protein-DNA binding structure is preserved because the histone is crosslinked before the cell is lysed. The protein and DNA are bound together even after the chromatin is broken up into smaller pieces. Then the protein-DNA conjugates are captured by immunoprecipitationwith antibodies binding specifically to regions in the protein of interest. Then the DNA is extracted from the proteins and analyzed by targeted qPCR or genome-wide next-generation sequencing (NGS).
New Call-to-action

Genomic DNA, nucleosomes, and histones

Genomic DNA is located inside the chromatin in the nucleus of the cell. The DNA is packaged into nucleosomes, which in turn are composed of histone cores that act as spools for the DNA. The histones are made of protein that binds the DNA through many different types of interactions including electrostatic, hydrogen-bonding, hydrophobic interactions, and dispersion forces. Non-histone proteins are also able to bind to the DNA through similar interactions. The secondary and tertiary folding structures and motifs of the proteins are very important to these interactions as certain binding pocket chemistries are only possible when the protein is folded properly. Histone and non-histone proteins bind to the DNA and alter the shape of the chromatin. The protein-DNA interactions govern how DNA unravels and re-spools around the histone. This controls which parts of the genome are unrolled and accessible for transcription. The CHIP assay is a good technique for evaluating this transcriptional regulation.

CHIP assay steps

The basic steps to performing a CHIP assay adapted from a ThermoFisher protocol:

  1. Crosslink the protein-DNA interactions
    1. Covalent interactions lock the proteins and DNA into place.
    2. Formaldehyde is commonly used but ethylene glycol bis(succinimidyl succinate)) (EGS) or disuccinimidylglutarate (DSG) can be used. The benefit of EGS or DSG is that they can preserve higher levels of protein-DNA interactions such as complexes where proteins are associated with DNA without being directly bound to it.
  2. Cell lysis
    1. Cell lysis buffers, salts, and detergents are commonly used to free the nuclear material from the cell.
    2. Mechanical disruption can be used if necessary
  3. Chromatin Shearing
    1. The native chromatin is too large to use for immunoprecipitation so it must be enzymatically digested into smaller pieces around 500 bp
  4. Immunoprecipitation
    1. Antibodies specific to the target protein(s) are used to isolate the protein-DNA complexes
    2. Agarose or magnetic beads coated with protein A/G are commonly used to immobilize antibodies to the bead surface for protein capture
  5. Reverse crosslinking to free the DNA from the target protein
    1. Heat is used to reverse crosslinks
    2. Proteinases are used to digest the protein
  6. Analysis of captured DNA
    1. To determine the genomic region that the target protein was associated with the captured DNA is analyzed by qPCR

 

Related news

 New Call-to-action

Leave a comment