The key parameter for the biomagnetic separation processes is the magnetic force applied over the magnetic beads' suspension. The competition of this force with the drag force generated by the buffer viscosity will translate into the speed at which the magnetic beads separate.
Magnetic DNA purification is a simple and reliable way to isolate DNA.
This method for purifying DNA has a wide range of applications. In fact, genomic sequencing and molecular analysis have become so integral to biological research that they are now all but required for work to be published in high profile journals.
A cell lysis buffer is a critical first component to any isolation protocol. It is fundamental to the first step of protein or nucleic acid extraction as it aids in the chemical breakdown of cell membranes and compartments, enabling target molecules to leave the cell. There are many types of lysis buffers; most are easy to make, but most are also commercially available. They are often included in kits for immunoprecipitation, co-ip protocol, nucleic acid isolation, and others. When using a lysis buffer for protein capture the addition of protease inhibitors is generally recommended in order to protect proteins.
Biomagnetic separation has a wide range of applications in life sciences, from cell sorting to protein purification. But we regularly speak to laboratories and companies whose magnetic separation protocols lack necessary information on the key parameter: magnetic force.
By improving the capture and isolation of biomolecules in complex matrices, magnetic beads have facilitated a leap forward in life science technologies..
We are excited to announce that we will be exhibiting at the 2022 AACC Clinical Lab Expo!
Visit our booth nr. 1559 to learn more on how to attain a safe and high-performing biomagnetic separation process. Discover how you can work with our systems with volumes from milliliters up to 50 liters, assuring batch consistency thanks to a constant magnetic force and an intuitive monitoring system.
Good manufacturing practice, or GMP, is a set of standards that ensures that produced products meet a set of quality standards. Following GMP is crucial for the production of laboratory equipment, as it ensures that a manufactured product is able to meet predefined criteria. Most GMP practices follow the guidelines set by the FDA in the United States, and are promoted by the WHO.
Our understanding of genetic material has substantially increased since Friederich Miescher first extracted DNA in 1869. He discovered that a material exists within cells that precipitates out of acidic solution and dissolves into alkaline solution. He called it nuclein because it seemed to be located within the nucleus. It took until 1953 for the structure of DNA to be elucidated. It was during this time that procedures to isolate DNA began to emerge. Later, during the 1960's and 70's scientists were furiously untangling the cellular environment, and the discovery of RNA with its various forms and functions further refined DNA purification procedures.
Isolating cell populations is required for many fields of research, such as cell function, signaling and gene expression. Techniques that enable the rapid and accurate enrichment of target cell populations are therefore an area of substantial interest.
Cell sorting techniques fall into two general categories:
- Bulk sorting
- Single cell sorting
In single cell sorting each cell is analyzed individually, whereas in bulk cell sorting all of the target cells are collected together.
While cell sorting is highly accurate, a sorted cell population is not “pure”. Instead, the collected population is referred to as “enriched”. Compared to bulk sorting, single cell sorting results in more homogeneous and highly enriched cell populations.
