Biodetection and biosensors are widely used for diagnosing disease or infection, point-of-care monitoring and treatment, detecting toxins, environmental monitoring, forensics and research. Biosensing technology has a crucial role to play in future biomedicine and healthcare. Biodetection is a broad term that encompasses the global strategies in place for the detection of biological threats such as pathogens, infectious diseases, and biological weapons. In particular, portable biosensing instruments such as lab-on-chip technologies are opening up new possibilities for biodetection systems identifying outbreaks of infectious pathogens.
Flow cytometry interpretation can be challenging, particularly when conducting multiparametric analyses that typically create a large quantity of data points. Fluorescent activated cell sorting (FACS), a derivative of flow cytometry, has become an essential tool for modern life science and biomedical laboratories. It is one of two main methods for cell sorting, alongside magnetic bead separation. FACS is a method for rapidly analyzing cells based on multiple criteria simultaneously, and then physically sorting them based on this analysis. The results of the process are usually represented by dot plots: two or three-dimensional scatter plots, or sometimes a histogram. However, these plots can be tricky to interpret, so here are a few tips to help get you started.
The key parameter for the biomagnetic separation processes is the magnetic force applied over the magnetic beads' suspension. The competition of this force with the drag force generated by the buffer viscosity will translate into the speed at which the magnetic beads separate.
A cell lysis buffer is a critical first component to any isolation protocol. It is fundamental to the first step of protein or nucleic acid extraction as it aids in the chemical breakdown of cell membranes and compartments, enabling target molecules to leave the cell. There are many types of lysis buffers; most are easy to make, but most are also commercially available. They are often included in kits for immunoprecipitation, co-ip protocol, nucleic acid isolation, and others. When using a lysis buffer for protein capture the addition of protease inhibitors is generally recommended in order to protect proteins.
Biomagnetic separation has a wide range of applications in life sciences, from cell sorting to protein purification. But we regularly speak to laboratories and companies whose magnetic separation protocols lack necessary information on the key parameter: magnetic force.
By improving the capture and isolation of biomolecules in complex matrices, magnetic beads have facilitated a leap forward in life science technologies..
We are excited to announce that we will be exhibiting at the 2022 AACC Clinical Lab Expo!
Visit our booth nr. 1559 to learn more on how to attain a safe and high-performing biomagnetic separation process. Discover how you can work with our systems with volumes from milliliters up to 50 liters, assuring batch consistency thanks to a constant magnetic force and an intuitive monitoring system.
Good manufacturing practice, or GMP, is a set of standards that ensures that produced products meet a set of quality standards. Following GMP is crucial for the production of laboratory equipment, as it ensures that a manufactured product is able to meet predefined criteria. Most GMP practices follow the guidelines set by the FDA in the United States, and are promoted by the WHO.
Our understanding of genetic material has substantially increased since Friederich Miescher first extracted DNA in 1869. He discovered that a material exists within cells that precipitates out of acidic solution and dissolves into alkaline solution. He called it nuclein because it seemed to be located within the nucleus. It took until 1953 for the structure of DNA to be elucidated. It was during this time that procedures to isolate DNA began to emerge. Later, during the 1960's and 70's scientists were furiously untangling the cellular environment, and the discovery of RNA with its various forms and functions further refined DNA purification procedures.
