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Customizable Nanoframeworks are one of the most exciting innovations in the world of nanochemistry. There are two main classifications of nanoframeworks. The first is the Metal-Organic framework (MOF). A MOG is a classification of a compound that consists of a metal linked to an organic ligand to form a coordinated structure in 1, 2 or 3 dimensions.

The second is a Covalent-Organic framework (COF), which is a crystalline porous organic framework with two or three dimensional properties. A COF is usually, but not always, limited to light elements (H, B, C, N and O) . Both possess a π-conjugated system and have a wide porous volume that can be tuned with the selection of a linker. This linker also has further effects on the electronic structure of the material. Thousands upon thousands of different, unique frameworks have been identified, leading to a variety of sizes that range from the nm to mm range. However, in all cases, the porosity of the framework benefits from a high surface area to volume ratio, leading to many different applications using a delivery mechanism that benefits from rapid diffusion. 

Sepmag starts distributing its unique Biomagnetic separation systems in India, where Biotechnology industry is fast developing. In Vitro Diagnostic, protein purification and cell sorting companies working with magnetic beads will benefit enormously from the quality, reliability and monitoring that Sepmag technology offers to biotech enterprises around the globe.

Traditionally, biomagnetic separation users have not monitored the separation process. The nature of classical separators, where the magnetic force changes with the distance, means you can determine when the separation is complete (the buffer becomes transparent), but it is difficult to interpret the optical changes during the process. This is because every location sampled will have a different bead concentration due to their different speeds. In addition, it is difficult to compare different batches as even a small difference in the vessel’s position within the separator will affect the beads’ behavior.

Batch adsorption with magnetic beads is considered a mild technique, which is advantageous for the purification of sensitive plasma proteins. Cytiva’s patent application shows excellent results using magnetic beads with suitable ligands for the purification of Factor VIII and von Willebrand Factor in solubilized cryoprecipitate, and Factor IX in cold supernatant. The magnetic separation system, with constant magnetic force, used in these experiments enables the straightforward transferring of the protocols to large-scale volumes.

The Importance of protein purification buffer

A buffer, by definition, resists changes in pH when small quantities of acid or base are added to it. Protein purification protocols utilize several types of buffers to aid proteins in your solution in binding to your separation mechanism, then washing out unnecessary molecules from the solution, and finally to elute the purified protein and store it. There are many types of buffers that come at different pH’s. Depending on need, scientists will use additives such as salt, as well as protease inhibitors to create the ideal protein purification buffer for their protein. Common Buffers are Tris-HCl, HEPES-NaOH, MOPS, etc. At the low end of the pH range, citric acid-NaOH can be used in the 2.2 to 6.5 pH range. MES-NaOH is closer to pH 6, while imidazole-HCl is around 7. Tris-HCl is up around pH 8 while HEPES-NaOH is between 7 and 8. Differences in pKa, the strength of the buffer, can arise from differences in temperature of the buffer. After using an elution buffer to elute your protein, you will want to quickly neutralize it with a storage buffer to keep the protein from experiencing damage.

The Annual 2023 Two-Day IVD Conference will take place on March 21-22, 2023, in Rome, Italy.

SEPMAG will participate and provide helpful insights on how to develop protocols based on the utilization of magnetic beads in manufacturing processes.

Immunoaffinity chromatography is a method for separating target antibodies or antigens from a heterogeneous solution. It is column-based, which means that the solution is flowed through a column and eluted at the other end. The column is pre-functionalized with the capture antibody or antigen. The target protein is adsorbed onto the resin-bound capture protein and is retained in the column while the remaining solution is eluted. The fraction containing the target protein is later eluted and purified.

Cube Biotech’s video explores magnetic beads as a solution for large-scale protein purification using a Sepmag A400ml.

RT-LAMP is a Loop-Mediated Isothermal Amplification (LAMP) assay that uses a reverse transcription enzyme.

Protein extraction is a key step for many proteomics research procedures, from ELISA to Western Blot. Proteins form the basis of all cells, tissue, and organisms. Proteins also initiate and mediate the thousands of biochemical pathways that govern an organism’s function. Biomedical studies of proteins can reveal information about pathways of disease, and the expression of the genetic code. But before proteins can be studied, they need to be extracted. Choosing the most appropriate protein extraction method is key to successful protein extraction.