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Posted on Tue, Feb 14, 2023

Protein Purification using Magnetic Beads: One Protocol, All Sample Sizes

Cube Biotech’s video explores magnetic beads as a solution for large-scale protein purification using a Sepmag A400ml.


Traditional column chromatography is a well-established technology for many protein purification processes. However, it has its limitations when working with large quantities of protein, the raw suspensions are dirty or with low-expressing proteins. In these cases, magnetic beads offer a simple and effective alternative; in the absence of a magnetic force, the mobility of the solid support allows the efficient isolation of the target protein from the cell lysate. Once the capture is complete, applying a magnetic force allows the immobilization of the beads (and the target protein) in a few seconds, and then the removal of the supernatant without the risk of clogging columns. The result is a fast, clean, and efficient process.

Free guide: 7 Keys to Successfully Scaling-up Biomagnetic Separation Processes

Mistakenly, scaling up the magnetic beads protein purification protocols has been considered difficult - if not impossible. Even if this were true when you use classical magnetic separators or simple magnets, this problem doesn’t exist when you use constant magnetic force separation systems.

By using simple magnets or classical magnetic separators, the magnetic force quickly decreases with the distance, limiting its reach, thus, the working volume. By contrast, modern Biomagnetic Separation Systems generate a constant magnetic force in all the working volume, overcoming the distance problem. Hence, magnetic beads can be used in large vessels while keeping all the benefits of magnetic separation at a large scale (fast process, no loss of material, easy buffer exchange, and no maintenance).

Chemiluminescent IVD reagent manufacturers faced the same problem 15 years ago when they needed to scale up the production batches in order to cope with the increasing demand for the high throughput of CLIA analyzers. Luckily, they found the solution in the modern Biomagnetic Separation Systems, such as SEPMAG Q or N. Today, they routinely work with bottles and carboys with up to 50 L magnetic beads suspension batches.

In a recent video, Cube Biotech, a company specializing in Protein Purification, explores magnetic beads as a solution for large-scale protein purification using a Sepmag A400ml Biomagnetic Separation System. The video provides valuable insights and techniques for large-scale protein purification using magnetic beads. We hope you found this enlighting and useful!


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