Blog https://www.sepmag.eu/blog en-us Fri, 02 Dec 2022 08:49:50 GMT 2022-12-02T08:49:50Z en-us Agarose Resin Chromatography https://www.sepmag.eu/blog/agarose-resin-chromatography <div class="hs-featured-image-wrapper"> <a href="https://www.sepmag.eu/blog/agarose-resin-chromatography" title="" class="hs-featured-image-link"> <img src="https://www.sepmag.eu/hubfs/quenchers.jpg" alt="quenchers" class="hs-featured-image" style="width:auto !important; max-width:50%; float:left; margin:0 15px 15px 0;"> </a> </div> <p style="text-align: justify;"><span style="color: #333333;">Using Agarose Resin chromatography allows for a versatile, prepacked column that enables <strong>small-scale, high-resolution size exclusion chromatography for preparation, characterization</strong>, and analysis of proteins and other biomolecules.</span></p> <div class="hs-featured-image-wrapper"> <a href="https://www.sepmag.eu/blog/agarose-resin-chromatography" title="" class="hs-featured-image-link"> <img src="https://www.sepmag.eu/hubfs/quenchers.jpg" alt="quenchers" class="hs-featured-image" style="width:auto !important; max-width:50%; float:left; margin:0 15px 15px 0;"> </a> </div> <p style="text-align: justify;"><span style="color: #333333;">Using Agarose Resin chromatography allows for a versatile, prepacked column that enables <strong>small-scale, high-resolution size exclusion chromatography for preparation, characterization</strong>, and analysis of proteins and other biomolecules.</span></p> <img src="https://track.hubspot.com/__ptq.gif?a=213437&amp;k=14&amp;r=https%3A%2F%2Fwww.sepmag.eu%2Fblog%2Fagarose-resin-chromatography&amp;bu=https%253A%252F%252Fwww.sepmag.eu%252Fblog&amp;bvt=rss" alt="" width="1" height="1" style="min-height:1px!important;width:1px!important;border-width:0!important;margin-top:0!important;margin-bottom:0!important;margin-right:0!important;margin-left:0!important;padding-top:0!important;padding-bottom:0!important;padding-right:0!important;padding-left:0!important; "> Fri, 02 Dec 2022 08:49:50 GMT https://www.sepmag.eu/blog/agarose-resin-chromatography 2022-12-02T08:49:50Z Lluis M. Martínez, SEPMAG Chief Scientific Officer What is the function of quenchers? https://www.sepmag.eu/blog/what-is-the-function-of-quenchers <div class="hs-featured-image-wrapper"> <a href="https://www.sepmag.eu/blog/what-is-the-function-of-quenchers" title="" class="hs-featured-image-link"> <img src="https://www.sepmag.eu/hubfs/quenchers.jpg" alt="quenchers" class="hs-featured-image" style="width:auto !important; max-width:50%; float:left; margin:0 15px 15px 0;"> </a> </div> <p style="text-align: justify;"><span style="color: #333333;"><strong>Quenchers are a substance that can decrease or end an effect or process. </strong>They are often used to absorb the excitation energy emitted by fluorophores (i.e., fluorescence), which signals that another process has taken place. Most re-emit much of the energy as visible light. Others, called dark quenchers, do not have native fluorescence and instead emit the energy as heat. Examples of common quenchers include iodide ions, molecular oxygen and acrylamide.. <br></span></p> <div class="hs-featured-image-wrapper"> <a href="https://www.sepmag.eu/blog/what-is-the-function-of-quenchers" title="" class="hs-featured-image-link"> <img src="https://www.sepmag.eu/hubfs/quenchers.jpg" alt="quenchers" class="hs-featured-image" style="width:auto !important; max-width:50%; float:left; margin:0 15px 15px 0;"> </a> </div> <p style="text-align: justify;"><span style="color: #333333;"><strong>Quenchers are a substance that can decrease or end an effect or process. </strong>They are often used to absorb the excitation energy emitted by fluorophores (i.e., fluorescence), which signals that another process has taken place. Most re-emit much of the energy as visible light. Others, called dark quenchers, do not have native fluorescence and instead emit the energy as heat. Examples of common quenchers include iodide ions, molecular oxygen and acrylamide.. <br></span></p> <img src="https://track.hubspot.com/__ptq.gif?a=213437&amp;k=14&amp;r=https%3A%2F%2Fwww.sepmag.eu%2Fblog%2Fwhat-is-the-function-of-quenchers&amp;bu=https%253A%252F%252Fwww.sepmag.eu%252Fblog&amp;bvt=rss" alt="" width="1" height="1" style="min-height:1px!important;width:1px!important;border-width:0!important;margin-top:0!important;margin-bottom:0!important;margin-right:0!important;margin-left:0!important;padding-top:0!important;padding-bottom:0!important;padding-right:0!important;padding-left:0!important; "> Thu, 24 Nov 2022 14:40:32 GMT https://www.sepmag.eu/blog/what-is-the-function-of-quenchers 2022-11-24T14:40:32Z Lluis M. Martínez, SEPMAG Chief Scientific Officer Different types of Immunoassays https://www.sepmag.eu/blog/different-types-of-immunoassays <div class="hs-featured-image-wrapper"> <a href="https://www.sepmag.eu/blog/different-types-of-immunoassays" title="" class="hs-featured-image-link"> <img src="https://www.sepmag.eu/hubfs/types%20of%20immunoassays.png" alt="types of immunoassays" class="hs-featured-image" style="width:auto !important; max-width:50%; float:left; margin:0 15px 15px 0;"> </a> </div> <div> <p style="text-align: justify;"><strong>Immunoassays are tests that detect the presence of a specific molecule in a sample using antibody-antigen binding reactions.</strong> Antibodies bind to the specific structure of a particular antigen, making immunoassays highly specific: the antibody will only bind to a specific structure of a particular antigen. This makes antibodies effective reagents for detecting target molecules. As a result, immunoassays are a fundamental tool for hospitals, life science research and industry laboratories. Immunoassays come in a range of formats, and can be used to assess disease, track proteins, and detect environmental contamination.</p> </div> <div class="hs-featured-image-wrapper"> <a href="https://www.sepmag.eu/blog/different-types-of-immunoassays" title="" class="hs-featured-image-link"> <img src="https://www.sepmag.eu/hubfs/types%20of%20immunoassays.png" alt="types of immunoassays" class="hs-featured-image" style="width:auto !important; max-width:50%; float:left; margin:0 15px 15px 0;"> </a> </div> <div> <p style="text-align: justify;"><strong>Immunoassays are tests that detect the presence of a specific molecule in a sample using antibody-antigen binding reactions.</strong> Antibodies bind to the specific structure of a particular antigen, making immunoassays highly specific: the antibody will only bind to a specific structure of a particular antigen. This makes antibodies effective reagents for detecting target molecules. As a result, immunoassays are a fundamental tool for hospitals, life science research and industry laboratories. Immunoassays come in a range of formats, and can be used to assess disease, track proteins, and detect environmental contamination.</p> </div> <img src="https://track.hubspot.com/__ptq.gif?a=213437&amp;k=14&amp;r=https%3A%2F%2Fwww.sepmag.eu%2Fblog%2Fdifferent-types-of-immunoassays&amp;bu=https%253A%252F%252Fwww.sepmag.eu%252Fblog&amp;bvt=rss" alt="" width="1" height="1" style="min-height:1px!important;width:1px!important;border-width:0!important;margin-top:0!important;margin-bottom:0!important;margin-right:0!important;margin-left:0!important;padding-top:0!important;padding-bottom:0!important;padding-right:0!important;padding-left:0!important; "> News Mon, 21 Nov 2022 08:34:10 GMT https://www.sepmag.eu/blog/different-types-of-immunoassays 2022-11-21T08:34:10Z Lluis M. Martínez, SEPMAG Chief Scientific Officer DBCO click chemistry: what is it and what are its benefits? https://www.sepmag.eu/blog/dbco-click-chemistry-what-is-it-and-benefits <div class="hs-featured-image-wrapper"> <a href="https://www.sepmag.eu/blog/dbco-click-chemistry-what-is-it-and-benefits" title="" class="hs-featured-image-link"> <img src="https://www.sepmag.eu/hubfs/dbco%20click%20chemistry.jpg" alt="dbco click chemistry" class="hs-featured-image" style="width:auto !important; max-width:50%; float:left; margin:0 15px 15px 0;"> </a> </div> <p><span style="text-decoration: underline;">DBCO click chemistry</span><span style="text-decoration: underline;"> is a type of reaction that has underpinned recent advances in biomedical research, as well as pharmaceuticals and biotech.</span> Copper(I)-free click chemistry, such as DBCO click reactions, <span style="font-weight: bold;">enable fast and specific conjugation of target molecules under aqueous conditions and do not require toxic cata</span><span style="color: #333333;"><span style="font-weight: bold;">lysts</span>. <br></span></p> <div class="hs-featured-image-wrapper"> <a href="https://www.sepmag.eu/blog/dbco-click-chemistry-what-is-it-and-benefits" title="" class="hs-featured-image-link"> <img src="https://www.sepmag.eu/hubfs/dbco%20click%20chemistry.jpg" alt="dbco click chemistry" class="hs-featured-image" style="width:auto !important; max-width:50%; float:left; margin:0 15px 15px 0;"> </a> </div> <p><span style="text-decoration: underline;">DBCO click chemistry</span><span style="text-decoration: underline;"> is a type of reaction that has underpinned recent advances in biomedical research, as well as pharmaceuticals and biotech.</span> Copper(I)-free click chemistry, such as DBCO click reactions, <span style="font-weight: bold;">enable fast and specific conjugation of target molecules under aqueous conditions and do not require toxic cata</span><span style="color: #333333;"><span style="font-weight: bold;">lysts</span>. <br></span></p> <img src="https://track.hubspot.com/__ptq.gif?a=213437&amp;k=14&amp;r=https%3A%2F%2Fwww.sepmag.eu%2Fblog%2Fdbco-click-chemistry-what-is-it-and-benefits&amp;bu=https%253A%252F%252Fwww.sepmag.eu%252Fblog&amp;bvt=rss" alt="" width="1" height="1" style="min-height:1px!important;width:1px!important;border-width:0!important;margin-top:0!important;margin-bottom:0!important;margin-right:0!important;margin-left:0!important;padding-top:0!important;padding-bottom:0!important;padding-right:0!important;padding-left:0!important; "> Mon, 07 Nov 2022 08:21:55 GMT https://www.sepmag.eu/blog/dbco-click-chemistry-what-is-it-and-benefits 2022-11-07T08:21:55Z Lluis M. Martínez, SEPMAG Chief Scientific Officer What is poly dt? https://www.sepmag.eu/blog/what-is-poly-dt <div class="hs-featured-image-wrapper"> <a href="https://www.sepmag.eu/blog/what-is-poly-dt" title="" class="hs-featured-image-link"> <img src="https://www.sepmag.eu/hubfs/poly%20dt.jpg" alt="poly dt" class="hs-featured-image" style="width:auto !important; max-width:50%; float:left; margin:0 15px 15px 0;"> </a> </div> <p style="text-align: justify;"><span style="font-weight: bold;">Poly deoxythymine</span> (poly dt) is a molecule made up of a <span style="font-weight: bold;">string of deoxythymidines</span> that are connected via 3' to 5' phosphodiester linkages.&nbsp;</p> <p style="text-align: justify;">In other words, poly dt is <span style="font-weight: bold;">a long chain of thymine nucleotides</span>, which can be <a href="https://www.sciencedirect.com/topics/neuroscience/polythymidylic-acid"><span>up to 20,000 nucleotides long</span></a>. Also known as polythymidine, polythymidylic acid, and poly T, amongst other names, poly dt is a single-stranded sequence commonly used to purify messenger RNA (mRNA)..</p> <div class="hs-featured-image-wrapper"> <a href="https://www.sepmag.eu/blog/what-is-poly-dt" title="" class="hs-featured-image-link"> <img src="https://www.sepmag.eu/hubfs/poly%20dt.jpg" alt="poly dt" class="hs-featured-image" style="width:auto !important; max-width:50%; float:left; margin:0 15px 15px 0;"> </a> </div> <p style="text-align: justify;"><span style="font-weight: bold;">Poly deoxythymine</span> (poly dt) is a molecule made up of a <span style="font-weight: bold;">string of deoxythymidines</span> that are connected via 3' to 5' phosphodiester linkages.&nbsp;</p> <p style="text-align: justify;">In other words, poly dt is <span style="font-weight: bold;">a long chain of thymine nucleotides</span>, which can be <a href="https://www.sciencedirect.com/topics/neuroscience/polythymidylic-acid"><span>up to 20,000 nucleotides long</span></a>. Also known as polythymidine, polythymidylic acid, and poly T, amongst other names, poly dt is a single-stranded sequence commonly used to purify messenger RNA (mRNA)..</p> <img src="https://track.hubspot.com/__ptq.gif?a=213437&amp;k=14&amp;r=https%3A%2F%2Fwww.sepmag.eu%2Fblog%2Fwhat-is-poly-dt&amp;bu=https%253A%252F%252Fwww.sepmag.eu%252Fblog&amp;bvt=rss" alt="" width="1" height="1" style="min-height:1px!important;width:1px!important;border-width:0!important;margin-top:0!important;margin-bottom:0!important;margin-right:0!important;margin-left:0!important;padding-top:0!important;padding-bottom:0!important;padding-right:0!important;padding-left:0!important; "> Thu, 27 Oct 2022 14:57:57 GMT https://www.sepmag.eu/blog/what-is-poly-dt 2022-10-27T14:57:57Z Lluis M. Martínez, SEPMAG Chief Scientific Officer Magnetic Cell Separator https://www.sepmag.eu/blog/magnetic-cell-separator <div class="hs-featured-image-wrapper"> <a href="https://www.sepmag.eu/blog/magnetic-cell-separator" title="" class="hs-featured-image-link"> <img src="https://www.sepmag.eu/hubfs/magnetic%20cell%20separator.jpg" alt="magnetic cell separator" class="hs-featured-image" style="width:auto !important; max-width:50%; float:left; margin:0 15px 15px 0;"> </a> </div> <p><strong>The magnetic cell separation technique:</strong> Executing cell separation by magnetic activation has been a trusted technique by scientists for decades. Cell sorting is ubiquitously used in research and clinical settings where a target cell of interest needs to be isolated from a heterogenous mixture such as serum or plasma. It is used in several scientific disciplines such as immunology, where it helps identify cells present during immune responses, or in cancer research elucidating tissue environment of tumors.</p> <div class="hs-featured-image-wrapper"> <a href="https://www.sepmag.eu/blog/magnetic-cell-separator" title="" class="hs-featured-image-link"> <img src="https://www.sepmag.eu/hubfs/magnetic%20cell%20separator.jpg" alt="magnetic cell separator" class="hs-featured-image" style="width:auto !important; max-width:50%; float:left; margin:0 15px 15px 0;"> </a> </div> <p><strong>The magnetic cell separation technique:</strong> Executing cell separation by magnetic activation has been a trusted technique by scientists for decades. Cell sorting is ubiquitously used in research and clinical settings where a target cell of interest needs to be isolated from a heterogenous mixture such as serum or plasma. It is used in several scientific disciplines such as immunology, where it helps identify cells present during immune responses, or in cancer research elucidating tissue environment of tumors.</p> <img src="https://track.hubspot.com/__ptq.gif?a=213437&amp;k=14&amp;r=https%3A%2F%2Fwww.sepmag.eu%2Fblog%2Fmagnetic-cell-separator&amp;bu=https%253A%252F%252Fwww.sepmag.eu%252Fblog&amp;bvt=rss" alt="" width="1" height="1" style="min-height:1px!important;width:1px!important;border-width:0!important;margin-top:0!important;margin-bottom:0!important;margin-right:0!important;margin-left:0!important;padding-top:0!important;padding-bottom:0!important;padding-right:0!important;padding-left:0!important; "> Thu, 20 Oct 2022 14:44:01 GMT https://www.sepmag.eu/blog/magnetic-cell-separator 2022-10-20T14:44:01Z Lluis M. Martínez, SEPMAG Chief Scientific Officer How to generate a constant magnetic force https://www.sepmag.eu/blog/how-to-generate-a-constant-magnetic-force <div class="hs-featured-image-wrapper"> <a href="https://www.sepmag.eu/blog/how-to-generate-a-constant-magnetic-force" title="" class="hs-featured-image-link"> <img src="https://www.sepmag.eu/hubfs/IC%20-%20Ebook%20-%20How%20to%20master%20your%20biomagnetic%20separation%20processes%20consistency%20and%20monitoring/SEP%20-%20Portada3D%20-%20Tag%20-%20How%20to%20master%20your%20biomagnetic%20separation%20processes_%20consistency%20and%20monitoring%20%281%29.jpg" alt="How to generate a constant magnetic force" class="hs-featured-image" style="width:auto !important; max-width:50%; float:left; margin:0 15px 15px 0;"> </a> </div> <p style="font-weight: bold;"><span style="font-weight: normal;">A constant magnetic force across the whole working volume is key to consistency in biomagnetic separation processes. This ensures that <span style="font-weight: bold;">all beads in the suspension experience the same force</span>. Classical magnetic separators can't provide these conditions because the magnetic force they generate decreases with distance.</span><br></p> <div class="hs-featured-image-wrapper"> <a href="https://www.sepmag.eu/blog/how-to-generate-a-constant-magnetic-force" title="" class="hs-featured-image-link"> <img src="https://www.sepmag.eu/hubfs/IC%20-%20Ebook%20-%20How%20to%20master%20your%20biomagnetic%20separation%20processes%20consistency%20and%20monitoring/SEP%20-%20Portada3D%20-%20Tag%20-%20How%20to%20master%20your%20biomagnetic%20separation%20processes_%20consistency%20and%20monitoring%20%281%29.jpg" alt="How to generate a constant magnetic force" class="hs-featured-image" style="width:auto !important; max-width:50%; float:left; margin:0 15px 15px 0;"> </a> </div> <p style="font-weight: bold;"><span style="font-weight: normal;">A constant magnetic force across the whole working volume is key to consistency in biomagnetic separation processes. This ensures that <span style="font-weight: bold;">all beads in the suspension experience the same force</span>. Classical magnetic separators can't provide these conditions because the magnetic force they generate decreases with distance.</span><br></p> <img src="https://track.hubspot.com/__ptq.gif?a=213437&amp;k=14&amp;r=https%3A%2F%2Fwww.sepmag.eu%2Fblog%2Fhow-to-generate-a-constant-magnetic-force&amp;bu=https%253A%252F%252Fwww.sepmag.eu%252Fblog&amp;bvt=rss" alt="" width="1" height="1" style="min-height:1px!important;width:1px!important;border-width:0!important;margin-top:0!important;margin-bottom:0!important;margin-right:0!important;margin-left:0!important;padding-top:0!important;padding-bottom:0!important;padding-right:0!important;padding-left:0!important; "> News Thu, 13 Oct 2022 14:04:51 GMT https://www.sepmag.eu/blog/how-to-generate-a-constant-magnetic-force 2022-10-13T14:04:51Z Lluis M. Martínez, SEPMAG Chief Scientific Officer Macs cell sorting: technology and advantages https://www.sepmag.eu/blog/bid/398782/magnetic-activated-cell-sorting-technology-and-advantages <div class="hs-featured-image-wrapper"> <a href="https://www.sepmag.eu/blog/bid/398782/magnetic-activated-cell-sorting-technology-and-advantages" title="" class="hs-featured-image-link"> <img src="https://www.sepmag.eu/hubfs/macs%20cell%20sorting.jpg" alt="macs cell sorting" class="hs-featured-image" style="width:auto !important; max-width:50%; float:left; margin:0 15px 15px 0;"> </a> </div> <div style="text-align: justify;"> <h2 style="font-size: 16px;">Quick Background on Cell Sorting/Cell Isolation</h2> <p style="text-align: justify;">Researchers use <strong>magnetic activated cell isolation,</strong> also known as <strong>macs cell sorting</strong> or macs cell separation, to <strong>enrich a specific cell type from a mixed population. </strong>Scientists or companies sort or isolate cells so they can study or grow colonies of a single type of cell. They may use that type of cell for a specific type of functional assay crucial to that cell type or they might be interested in stem cells. Many labs use flow cytometry, also known as FACS (fluorescence activated cell sorting). This is a specialized and expensive technique that often resides in a core facility at a research institution. MACS has emerged as a cheaper alternative for cell sorting. Both these technologies use the highly specific antibody-antigen interaction to probe cells by their surface antigens by their specific antibody. Magnetic bead cell isolation has a more simple protocol and components than FACS, the process is briefly described below.&nbsp;</p> </div> <div class="hs-migrated-cms-post" style="text-align: justify;"></div> <div class="hs-featured-image-wrapper"> <a href="https://www.sepmag.eu/blog/bid/398782/magnetic-activated-cell-sorting-technology-and-advantages" title="" class="hs-featured-image-link"> <img src="https://www.sepmag.eu/hubfs/macs%20cell%20sorting.jpg" alt="macs cell sorting" class="hs-featured-image" style="width:auto !important; max-width:50%; float:left; margin:0 15px 15px 0;"> </a> </div> <div style="text-align: justify;"> <h2 style="font-size: 16px;">Quick Background on Cell Sorting/Cell Isolation</h2> <p style="text-align: justify;">Researchers use <strong>magnetic activated cell isolation,</strong> also known as <strong>macs cell sorting</strong> or macs cell separation, to <strong>enrich a specific cell type from a mixed population. </strong>Scientists or companies sort or isolate cells so they can study or grow colonies of a single type of cell. They may use that type of cell for a specific type of functional assay crucial to that cell type or they might be interested in stem cells. Many labs use flow cytometry, also known as FACS (fluorescence activated cell sorting). This is a specialized and expensive technique that often resides in a core facility at a research institution. MACS has emerged as a cheaper alternative for cell sorting. Both these technologies use the highly specific antibody-antigen interaction to probe cells by their surface antigens by their specific antibody. Magnetic bead cell isolation has a more simple protocol and components than FACS, the process is briefly described below.&nbsp;</p> </div> <div class="hs-migrated-cms-post" style="text-align: justify;"></div> <img src="https://track.hubspot.com/__ptq.gif?a=213437&amp;k=14&amp;r=https%3A%2F%2Fwww.sepmag.eu%2Fblog%2Fbid%2F398782%2Fmagnetic-activated-cell-sorting-technology-and-advantages&amp;bu=https%253A%252F%252Fwww.sepmag.eu%252Fblog&amp;bvt=rss" alt="" width="1" height="1" style="min-height:1px!important;width:1px!important;border-width:0!important;margin-top:0!important;margin-bottom:0!important;margin-right:0!important;margin-left:0!important;padding-top:0!important;padding-bottom:0!important;padding-right:0!important;padding-left:0!important; "> magnetic activated cell sorting Thu, 13 Oct 2022 12:13:37 GMT https://www.sepmag.eu/blog/bid/398782/magnetic-activated-cell-sorting-technology-and-advantages 2022-10-13T12:13:37Z Lluis M. Martínez, SEPMAG Chief Scientific Officer Biodetection and biosensing: technology and applications https://www.sepmag.eu/blog/biodetection-and-biosensing <div class="hs-featured-image-wrapper"> <a href="https://www.sepmag.eu/blog/biodetection-and-biosensing" title="" class="hs-featured-image-link"> <img src="https://www.sepmag.eu/hubfs/Biodetection.jpg" alt="Biodetection and biosensing" class="hs-featured-image" style="width:auto !important; max-width:50%; float:left; margin:0 15px 15px 0;"> </a> </div> <div class="hs-migrated-cms-post"> <p style="text-align: justify;"><span style="background-color: #ffffff;"><strong>Biodetection and biosensors</strong><strong> are widely used for</strong><span style="text-decoration: none;"><span style="color: #f06916;"><a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4986445/" style="background-color: #ffffff; color: #f06916;"> </a></span><span style="color: #f06916;"><a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4986445/" style="background-color: #ffffff; color: #f06916;"><strong>diagnosing disease or infection</strong></a></span></span><strong>, point-of-care monitoring and treatment, detecting toxins, environmental monitoring, forensics and research.</strong> Biosensing technology has a crucial role to play in future biomedicine and healthcare. Biodetection is a broad term that encompasses the global strategies in place for the detection of biological threats such as pathogens, infectious diseases, and biological weapons. In particular, portable biosensing instruments such as lab-on-chip technologies are opening up new possibilities for biodetection systems identifying outbreaks of infectious pathogens.</span></p> </div> <div class="hs-featured-image-wrapper"> <a href="https://www.sepmag.eu/blog/biodetection-and-biosensing" title="" class="hs-featured-image-link"> <img src="https://www.sepmag.eu/hubfs/Biodetection.jpg" alt="Biodetection and biosensing" class="hs-featured-image" style="width:auto !important; max-width:50%; float:left; margin:0 15px 15px 0;"> </a> </div> <div class="hs-migrated-cms-post"> <p style="text-align: justify;"><span style="background-color: #ffffff;"><strong>Biodetection and biosensors</strong><strong> are widely used for</strong><span style="text-decoration: none;"><span style="color: #f06916;"><a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4986445/" style="background-color: #ffffff; color: #f06916;"> </a></span><span style="color: #f06916;"><a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4986445/" style="background-color: #ffffff; color: #f06916;"><strong>diagnosing disease or infection</strong></a></span></span><strong>, point-of-care monitoring and treatment, detecting toxins, environmental monitoring, forensics and research.</strong> Biosensing technology has a crucial role to play in future biomedicine and healthcare. Biodetection is a broad term that encompasses the global strategies in place for the detection of biological threats such as pathogens, infectious diseases, and biological weapons. In particular, portable biosensing instruments such as lab-on-chip technologies are opening up new possibilities for biodetection systems identifying outbreaks of infectious pathogens.</span></p> </div> <img src="https://track.hubspot.com/__ptq.gif?a=213437&amp;k=14&amp;r=https%3A%2F%2Fwww.sepmag.eu%2Fblog%2Fbiodetection-and-biosensing&amp;bu=https%253A%252F%252Fwww.sepmag.eu%252Fblog&amp;bvt=rss" alt="" width="1" height="1" style="min-height:1px!important;width:1px!important;border-width:0!important;margin-top:0!important;margin-bottom:0!important;margin-right:0!important;margin-left:0!important;padding-top:0!important;padding-bottom:0!important;padding-right:0!important;padding-left:0!important; "> streptavidin beads Tue, 27 Sep 2022 11:43:12 GMT https://www.sepmag.eu/blog/biodetection-and-biosensing 2022-09-27T11:43:12Z Lluis M. Martínez, SEPMAG Chief Scientific Officer Flow Cytometry Interpretation: how to know what your results mean https://www.sepmag.eu/blog/flow-cytometry-interpretation <div class="hs-featured-image-wrapper"> <a href="https://www.sepmag.eu/blog/flow-cytometry-interpretation" title="" class="hs-featured-image-link"> <img src="https://www.sepmag.eu/hubfs/flow%20cytometry%20interpretation.jpg" alt="flow cytometry interpretation" class="hs-featured-image" style="width:auto !important; max-width:50%; float:left; margin:0 15px 15px 0;"> </a> </div> <p style="text-align: justify;"><strong>Flow cytometry interpretation</strong><strong> can be challenging, particularly when conducting multiparametric analyses that typically create a large quantity of data points.</strong> Fluorescent activated cell sorting (FACS), a derivative of flow cytometry, has become an essential tool for modern life science and biomedical laboratories. It is one of two main methods for cell sorting, alongside<span style="color: #f06916;"><a href="https://www.sepmag.eu/blog/bid/264475/centrifugation-and-filtering-with-biomagnetic-separation" style="text-decoration: none; color: #f06916;"> magnetic bead separation</a></span>. FACS is a method for rapidly analyzing cells based on multiple criteria simultaneously, and then physically sorting them based on this analysis. The results of the process are usually represented by dot plots: two or three-dimensional scatter plots, or sometimes a histogram. However, these plots can be tricky to interpret, so here are a few tips to help get you started.</p> <div class="hs-featured-image-wrapper"> <a href="https://www.sepmag.eu/blog/flow-cytometry-interpretation" title="" class="hs-featured-image-link"> <img src="https://www.sepmag.eu/hubfs/flow%20cytometry%20interpretation.jpg" alt="flow cytometry interpretation" class="hs-featured-image" style="width:auto !important; max-width:50%; float:left; margin:0 15px 15px 0;"> </a> </div> <p style="text-align: justify;"><strong>Flow cytometry interpretation</strong><strong> can be challenging, particularly when conducting multiparametric analyses that typically create a large quantity of data points.</strong> Fluorescent activated cell sorting (FACS), a derivative of flow cytometry, has become an essential tool for modern life science and biomedical laboratories. It is one of two main methods for cell sorting, alongside<span style="color: #f06916;"><a href="https://www.sepmag.eu/blog/bid/264475/centrifugation-and-filtering-with-biomagnetic-separation" style="text-decoration: none; color: #f06916;"> magnetic bead separation</a></span>. FACS is a method for rapidly analyzing cells based on multiple criteria simultaneously, and then physically sorting them based on this analysis. The results of the process are usually represented by dot plots: two or three-dimensional scatter plots, or sometimes a histogram. However, these plots can be tricky to interpret, so here are a few tips to help get you started.</p> <img src="https://track.hubspot.com/__ptq.gif?a=213437&amp;k=14&amp;r=https%3A%2F%2Fwww.sepmag.eu%2Fblog%2Fflow-cytometry-interpretation&amp;bu=https%253A%252F%252Fwww.sepmag.eu%252Fblog&amp;bvt=rss" alt="" width="1" height="1" style="min-height:1px!important;width:1px!important;border-width:0!important;margin-top:0!important;margin-bottom:0!important;margin-right:0!important;margin-left:0!important;padding-top:0!important;padding-bottom:0!important;padding-right:0!important;padding-left:0!important; "> quality control recording monitoring biomagnetic separation processes Thu, 08 Sep 2022 12:49:39 GMT https://www.sepmag.eu/blog/flow-cytometry-interpretation 2022-09-08T12:49:39Z Lluis M. Martínez, SEPMAG Chief Scientific Officer