An article by Sepmag founder and CSO, Dr. Lluis M. Martinez, appears online this week in Genetic Engineering & Biotechnology News. The article addresses issues thatariseduring a biomagnetic separation application and offers critical suggestions for approaching a process. Of particular importance is an understanding of the inherent parameters of a separation.
Merck Millipore has invited experts from around the globe to attend the symposium it has organised at the Hilton Hotel in Shanghai on May 15 and 16. The event will include contributors from China, as well as Germany, New Zealand, Russia, Spain and USA.
Microalgae become the exclusive focus in research of biofuel production to meet global energy demand. Photosynthetic microalgae use the sunlight to form biomass from the supplement of carbon dioxide and water. One of the main constituents of microalgal biomass is the natural oil stored within the cells. This natural oil can be further transformed into biodiesel through a transesterification process. The biofuel is renewable with huge potential to replace the fossil fuel. The International Energy Agency has reported that the total final oil consumption of the world in 2010 has reached 3575 Mtoe.1
When new magnetic beads reach the market, one of the questions users have is, how well will it separate?
When one scales up production using a classic magnetic separation system, one finds that the separation time increases quickly with an increase in production volume. An increase in separation time means that material losses are higher and aggregation problems become a serious problem. By using homogenous separation time, one finds that the magnetic separation process is shorter and the separation time can be easily estimated. In homogeneous systems material loss and bead aggregation is minimized.
In the Life Sciences, one of the most critical parameters for final IVD kit performance is magnetic bead concentration. The beads are functionalized before the magnetic separation process with antibodies or other biological molecules, so the concentration of magnetic beads also delivers a specific concentration of biologically active reagent. If you do not have the correct amount of beads/biological molecules in your preparation, the sensitivity of the kit changes significantly. Therefore volume control of the suspension is quite important.
In non-homogenous magnetic separators, monitoring the entire separation process is difficult to impossible. As a result, errors in the magnetic separation process, such as using the wrong magnetic beads or using buffers with the wrong properties are not detected until the final QC testing stage.
When magnetic bead reagents are produced in quantity, often you cannot know if you have the correct properties of the beads until the final quality control step. But if these properties are wrong, finding out the properties at the end of the magnetic separation process for production does not allow you to salvage the lot. Knowing magnetic bead properties, such as size, magnetic charge and surface charge, is critical in order to have excellent reproducibility in the final product (e.g. IVD kits).
When a lab has finally optimized their production process, they often link their process to a very specific piece of equipment and, by extension, have locked themselves into a constant volume. Often a lab develops its magnetic separation process for production with a specific magnetic separation device – this is normal. Usually the only parameter that needs to be adjusted during production is the separation time.
The separation time in standard magnetic separation devices is usually determined by analyzing aliquots of solution taken at different times. The problem is that each aliquot gives the technician information about one spatial point in time. Therefore, the design of validation experiments becomes a very complex endeavor.