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Agarose Resin Chromatography

Introduction to Chemiluminescence immunoassays

Using Agarose Resin chromatography allows for a versatile, prepacked column that enables small-scale, high-resolution size exclusion chromatography for preparation, characterization, and analysis of proteins and other biomolecules. Size exclusion chromatography, also known as gel filtration, is a common technique used to separate compounds of small molecules, such as proteins, polysaccharides, and nucleic acids when in an aqueous solution. This can be extremely useful in numerous commercial applications, such as analysis and determination of an unknown sample, removal of large proteins for purification of a sample, for removing small molecules such as dyes and primers, and buffer exchanges. Such chromatography is purchased as a prepacked column that allows for increased resolution and analysis, quick runtime, with a high pH tolerance to allow applications to a variety of molecules and substances. One example of a commercially available separation is Superose 6 chromatography, found here.

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Monitoring Biomagnetic Separation Processes: Sepmag® launches QCRv2

The traditional way to check whether a biomagnetic separation process is complete is by sight. The technician/researcher looks at the suspension: at the beginning of the process, the suspension is homogenous and opaque, but when the separation process is complete, the magnetic beads are left on the walls of the vessel and the supernatant is transparent. When the suspension is ‘transparent’, the technician stops the process by extracting the supernatant, leaving the magnetic beads in the bottle.

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Perfecting the magnetic separation process in magnetic bead IVD kit production

Magnetic bead kits are widely used in bioscience laboratories nowadays, as their development and perfecting during recent years has been significant. Both scientists in research labs, for immunoassays and magnetic separation processes, and lab technicians in hospitals, using IVD kits for molecular diagnostics, have experienced an improvement in their separation processes due to these magnetic bead kits. As using a biomagnetic separation process has proven useful for these applications, the increasing demand in magnetic beads has lead providers to develop cost-effective ways for their production.

Download our FREE guide about Biomagnetic Separation for Production HERE
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Will IKERLAT superparamagnetic latex microspheres work with Sepmag® Systems?

When new magnetic beads reach the market, one of the questions users have is, how well will it separate?

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Resuspension problems during the biomagnetic separation process

End product variability is caused in large part by resuspension problems in your process. During a magnetic separation process, the retention forces in the system need to be high enough that losses of the beads will be avoided when the buffer is pumped out. But the forces should not be too great because if they are excessive, beads will aggregate.

Download our FREE guide about Biomagnetic Separation for Production HERE
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The Safe Use of Biomagnetic Separation Systems in Production Facilities

Large magnetic systems create potentially high risk environments in terms of safety during a magnetic separation process. These systems generate large magnetic fields which result in the generation of large stray fields. The stray fields affect the surrounding environment and can attract ferromagnetic objects, potentially injuring system operators. The larger the system, the higher the risk, especially if workers do not follow Health and Safety protocols.

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The Effect of Magnetic Bead Concentration in Biomagnetic Separation

The concentration of magnetic beads is an important step in a magnetic separation process. Separation time is dependent on magnetic bead concentration, and final kit performance is also very dependent on accurate concentrating techniques, but liquid handling inaccuracies can lead to serious errors. If these concentration errors are not detected early in the process, excessive time, money and effort will need to be spent to either correct or redo the batch.

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New paper about the behavior of magnetic beads separation

Learning more about the behavior of magnetic beads separation when strong magnetic fields are applied

 

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Determining the Right Separation Time during Biomagnetic Separation Processes

When one scales up production using a classic magnetic separation system, one finds that the separation time increases quickly with an increase in production volume. An increase in separation time means that material losses are higher and aggregation problems become a serious problem. By using homogenous separation time, one finds that the magnetic separation process is shorter and the separation time can be easily estimated. In homogeneous systems material loss and bead aggregation is minimized. 

Download our FREE guide about Biomagnetic Separation for Production HERE
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How do concentration changes affect Biomagnetic Separation Processes?

In the Life Sciences, one of the most critical parameters for final IVD kit performance is magnetic bead concentration. The beads are functionalized before the magnetic separation process with antibodies or other biological molecules, so the concentration of magnetic beads also delivers a specific concentration of biologically active reagent. If you do not have the correct amount of beads/biological molecules in your preparation, the sensitivity of the kit changes significantly. Therefore volume control of the suspension is quite important.

Download our FREE guide about Biomagnetic Separation for Production HERE
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