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Why you can’t simply use a larger magnet for larger volumes

The key parameter for the biomagnetic separation processes is the magnetic force applied over the magnetic beads' suspension. The competition of this force with the drag force generated by the buffer viscosity will translate into the speed at which the magnetic beads separate.

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Cell Lysis Buffer

A cell lysis buffer is a critical first component to any isolation protocol. It is fundamental to the first step of protein or nucleic acid extraction as it aids in the chemical  breakdown of cell membranes and compartments, enabling target molecules to leave the cell. There are many types of lysis buffers; most are easy to make, but most are also commercially available. They are often included in kits for  immunoprecipitation, co-ip protocol, nucleic acid isolation, and others. When using a lysis buffer for protein capture the addition of protease inhibitors is generally recommended in order to protect proteins.

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Understanding magnetic force as the key parameter for biomagnetic separation processes

Biomagnetic separation has a wide range of applications in life sciences, from cell sorting to protein purification. But we regularly speak to laboratories and companies whose magnetic separation protocols lack necessary information on the key parameter: magnetic force.

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How to master your biomagnetic separation processes: consistency and monitoring

By improving the capture and isolation of biomolecules in complex matrices, magnetic beads have facilitated a leap forward in life science technologies..

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New e-book! How to master your biomagnetic separation processes

We are glad to announce the publication of a new e-book! Discover how you can take full advantage of this quick, efficient, and versatile technology.

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Meet with SEPMAG at AACC 2022!

We are excited to announce that we will be exhibiting at the 2022 AACC Clinical Lab Expo!

Visit our booth nr. 1559 to learn more on how to attain a safe and high-performing biomagnetic separation process. Discover how you can work with our systems with volumes from milliliters up to 50 liters, assuring batch consistency thanks to a constant magnetic force and an intuitive monitoring system.

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Nucleic Acid Isolation

Our understanding of genetic material has substantially increased since Friederich Miescher first extracted DNA in 1869. He discovered that a material exists within cells that precipitates out of acidic solution and dissolves into alkaline solution. He called it nuclein because it seemed to be located within the nucleus. It took until 1953 for the structure of DNA to be elucidated. It was during this time that procedures to isolate DNA began to emerge. Later, during the 1960's and 70's scientists were furiously untangling the cellular environment, and the discovery of RNA with its various forms and functions further refined DNA purification procedures.

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Cell sorting techniques

Isolating cell populations is required for many fields of research, such as cell function, signaling and gene expression. Techniques that enable the rapid and accurate enrichment of target cell populations are therefore an area of substantial interest.

Cell sorting techniques fall into two general categories: bulk sorting and single cell sorting. In single cell sorting each cell is analyzed individually, whereas in bulk cell sorting all of the target cells are collected together.

While cell sorting is highly accurate, a sorted cell population is not “pure”. Instead, the collected population is referred to as “enriched”. Compared to bulk sorting, single cell sorting results in more homogeneous and highly enriched cell populations.

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Nanobeads in biotechnology

Nanobeads have applications ranging from basic science research to clinical imaging and targeted drug delivery. Nanobeads are composites of nanoparticles. Nanoparticles are defined as being less than 100 nanometers in diameter, while nanobeads are usually around 50 to 200 nanometers in diameter. There are also microbeads, but these are much larger and have diameters of at least 1000 nanometers, or 1 micrometer, which is close to the size of a cell. Bacterial cell diameters range from 0.5 to 2 micrometers in diameter, and animal cells range from 10 to 30 micrometers in diameter. The size of nanobeads is very important to their function; partly because they are so much smaller than a cell, which enables them to be used for cell labeling and isolation. In the case of magnetic nanobeads, the nanometer size imparts the paramagnetic property that is so valuable for biomagnetic separation, clinical imaging (contrast enhanced magnetic resonance (MRI)), and therapeutics such as magnetic hyperthermia for targeted tumor destruction.

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A general filtration process

Filtration is a simple technique used to separate solid particles from suspension in a liquid solution. There are many filtration methods available, but all are based on the same general principle: a heterogenous mixture is poured over a filter membrane. The filter membrane has pores of a particular size. Particles larger than the pores will be unable to pass through the membrane, while particles smaller than the pores will pass through unhindered. Additionally, all liquids will pass through. The final result of a filtration process is a collection of residue on the filtration membrane. This residue is therefore effectively separated from the rest of the mixture that passed through the membrane.

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