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Immunomagnetic cell separation

Isolation and detection of a target molecule in cell therapeutics from a sample with high background debris or unwanted moleculesisa challenging task. Immunomagnetic-based separation is the most feasible technique to overcome the problems that come with the separation of cells and biomolecules from a complex matrix.

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Magnetic Nanoparticle Fluorescent Imaging

A fluorescent nanoparticle is a small particle containing a fluorophore that can be used to label biological material, such as a specific cell or tissue under fluorescence imaging. There are generally two locations for the particles to probe: ones that bind to the surface and others that bind internally. A large array of different nanoparticles can be used to achieve this, including but not limited to fluorescently doped silicas and sol-gels, hydrogels, hydrophobic organic polymers, and quantum dots. There are currently three main techniques for fluorescent problem.

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High molecular weight DNA: extraction using magnetic beads

Long read sequencing is making chromosome-scale assemblies, including diploid genomes, possible and is therefore improving our understanding of human genetic variation. But rapid improvements in long read sequencing capacity have been limited by the extraction of high molecular weight DNA. Magnetic bead-based high molecular weight DNA extraction limits DNA fragmentation, and is also less laborious and more cost-effective than other methods.

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Protein purification system

Proteins are large, complex biomolecules that perform a vast range of vital molecular functions in living organisms. Studies of the structure and function of proteins are helping to advance understanding of biology, but before proteins can be studied, they need to be isolated (i.e., purified). The best protein purification system for your application depends on the desired throughput, scale, and downstream application.

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Scaling up – common problems

The variation in magnetic force with distance when using classical magnetic separators is rarely problematic at small volumes. The short distance between the farthest beads and the magnet means that even with the mild magnetic forces generated by a small permanent magnet, separation time is fast and the efficiency is high.

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Different types of Immunoassays

Immunoassays are tests that detect the presence of a specific molecule in a sample using antibody-antigen binding reactions. Antibodies bind to the specific structure of a particular antigen, making immunoassays highly specific: the antibody will only bind to a specific structure of a particular antigen. This makes antibodies effective reagents for detecting target molecules. As a result, immunoassays are a fundamental tool for hospitals, life science research and industry laboratories. Immunoassays come in a range of formats, and can be used to assess disease, track proteins, and detect environmental contamination.

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The industrial centrifuge

The industrial centrifuge plays an integral role in the production of more things than one would initially expect. It is a commonly used tool in the food and agricultural sector, At pharmaceutical and biotechnology companies, for environmental management, and in the chemical industry. The word industry conjures up images of combination and creation—adding materials together to produce a final product. However, the separation of materials is just as important as the combination of materials. We can't create a new product until we have pure reactants to work with. This is especially important in the pharmaceutical and biotechnological realms, where reactant purity is essential to the production of a product that is safe for human consumption. This is where the centrifuge comes in. The centrifuge is used to separate heterogeneous mixtures into components varying by density.

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How to generate a constant magnetic force

A constant magnetic force across the whole working volume is key to consistency in biomagnetic separation processes. This ensures that all beads in the suspension experience the same force. Classical magnetic separators can't provide these conditions because the magnetic force they generate decreases with distance.

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Why you can’t simply use a larger magnet for larger volumes

The key parameter for the biomagnetic separation processes is the magnetic force applied over the magnetic beads' suspension. The competition of this force with the drag force generated by the buffer viscosity will translate into the speed at which the magnetic beads separate.

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Cell Lysis Buffer

A cell lysis buffer is a critical first component to any isolation protocol. It is fundamental to the first step of protein or nucleic acid extraction as it aids in the chemical  breakdown of cell membranes and compartments, enabling target molecules to leave the cell. There are many types of lysis buffers; most are easy to make, but most are also commercially available. They are often included in kits for  immunoprecipitation, co-ip protocol, nucleic acid isolation, and others. When using a lysis buffer for protein capture the addition of protease inhibitors is generally recommended in order to protect proteins.

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